More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| myocyte enhancer factor 2A | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human MEF2A(358-374aa MLSLGQVSAWQQHHLGQ), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MEF2A |
Protein | Myocyte-specific enhancer factor 2A |
Uniprot ID | Q02078 |
Function | Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle- specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter. |
Tissue Specificity | Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the MEF2 family. |
Aliases | ADCAD1 antibody|MADS box transcription enhancer factor 2 polypeptide A antibody|MADS box transcription enhancer factor 2, polypeptide A(myocyte enhancer factor 2A) antibody|MEF 2A antibody|MEF2 antibody|MEF2A antibody|MEF2A_HUMAN antibody|Myocyte enhancer factor 2A antibody|Myocyte-specific enhancer factor 2A antibody|RSRFC4 antibody|RSRFC9 antibody|Serum response factor like protein 1 antibody|Serum response factor-like protein 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MEF2A antibody, ASA-B1249, Western blotting
All lanes: Anti MEF2A (ASA-B1249) at 0.5ug/ml
Lane 1: Rat Heart Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: HT1080Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 55KD
Observed bind size: 55KD
All lanes: Anti MEF2A (ASA-B1249) at 0.5ug/ml
Lane 1: Rat Heart Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: HT1080Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 55KD
Observed bind size: 55KD
