More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| chemokine (C-C motif) ligand 2 | Polyclonal | IgG | Rabbit | Human | ELISA, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E. coli-derived human MCP1 recombinant protein (Position: Q24-T99). Human MCP1 shares 60.9% and 59.4% amino acid (aa) sequence identity with mouse and rat MCP1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CCL2 |
Protein | C-C motif chemokine 2 |
Uniprot ID | P13500 |
Function | Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the intercrine beta (chemokine CC) family. |
Aliases | C-C motif chemokine 2 antibody|CCL 2 antibody|CCL2 antibody|CCL2_HUMAN antibody|Chemokine (C C motif) ligand 2 antibody|Chemokine C C motif ligand 2 antibody|Chemokine CC Motif Ligand 2 antibody|GDCF 2 antibody|GDCF 2 HC11 antibody|GDCF-2 antibody|GDCF2 antibody|HC11 antibody|HSMCR30 antibody|HSMCR30 antibody|JE antibody|MCAF antibody|MCP 1 antibody|MCP-1 antibody|MCP1 antibody| MGC9434 antibody|Monocyte chemoattractant protein 1 antibody|Monocyte chemotactic and activating factor antibody|Monocyte chemotactic and activating factor antibody|Monocyte chemotactic protein 1 antibody|Monocyte secretory protein JE antibody|SCYA2 antibody|Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody|Small inducible cytokine A2 antibody|Small inducible cytokine subfamily A (Cys Cys), member 2 antibody|Small inducible cytokine subfamily A Cys Cys member 2 antibody|Small-inducible cytokine A2 antibody|SMC CF antibody|SMC-CF antibody|SMCCF antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- MCP1 antibody, ASA-B1242, Western blotting
All lanes: Anti MCP1 (ASA-B1242) at 0.5ug/ml
WB: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 11KD
Observed bind size: 11KD
All lanes: Anti MCP1 (ASA-B1242) at 0.5ug/ml
WB: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 11KD
Observed bind size: 11KD
