More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| minichromosome maintenance complex component 7 | Monoclonal | IgG | Mouse | Human | ICC, WB | Ascites |
Immunogen |
| Recombinant human Mcm7. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MCM7 |
Protein | DNA replication licensing factor MCM7 |
Uniprot ID | Human(P33993), Mouse(Q61881), Rat(Q6AYN8) |
Function | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. |
Tissue Specificity | |
Sub-cellular localization | Nucleus . |
Sequence Similarities | Belongs to the MCM family. |
Aliases | CDC47|MCM2|P1.1-MCM3|P85MCM|P1CDC47|PNAS146|CDC47, S. CEREVISIAE, HOMOLOG OF|MCM7 minichromosome maintenance deficient 7(S. cerevisiae)|minichromosome maintenance deficient(S. cerevisiae) 7|MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 7 |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.5-1μg/ml | Human | AssaySolutio's ECL kit |
| Immunocytochemistry | Suitable | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for ICC. *Blocking peptide can be purchased at $65. Contact us for more information
