More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| minichromosome maintenance complex component 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human MCM4(815-829aa TPALKYQQLFEDIRG), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MCM4 |
Protein | DNA replication licensing factor MCM4 |
Uniprot ID | P33991 |
Function | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. |
Tissue Specificity | |
Sub-cellular localization | Nucleus . |
Sequence Similarities | Belongs to the MCM family. |
Aliases | 19G antibody|AI325074 antibody|AU045576 antibody|CDC 21 antibody|CDC 54 antibody|CDC21 antibody|CDC21 homolog antibody|CDC21, S. pombe, homolog of antibody|CDC54 antibody|Cell division cycle 21, S. pombe, homolog of antibody|DNA replication licensing factor MCM 4 antibody|DNA replication licensing factor MCM4 antibody|hCdc 21 antibody|hCdc21 antibody|Homolog of S. pombe cell devision cycle 21 antibody|KIAA4003 antibody|mcdc21 antibody|MCM 4 antibody|MCM 4 minichromosome maintenance deficient 4 antibody|MCM4 antibody|MCM4 minichromosome maintenance deficient 4 antibody|MCM4 minichromosome maintenance deficient 4, mitotin(S. cerevisiae) antibody|MCM4_HUMAN antibody|Mcmd4 antibody|MGC33310 antibody|Minichromosome Maintenance 4 antibody|Minichromosome maintenance complex component 4 antibody|Minichromosome maintenance deficient(S. cerevisiae) 4 antibody|Minichromosome maintenance deficient 4 antibody|Minichromosome maintenance deficient 4 homolog(S. cerevisiae) antibody|Minichromosome maintenance, S. cerevisiae, homolog of, 4 antibody|mKIAA4003 antibody|P1 CDC21 antibody|P1-CDC21 antibody|P1CDC21 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MCM4 antibody, ASA-B1236, Western blotting
Lane 1: PANC Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: U20S Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 1: PANC Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: U20S Cell Lysate
Lane 4: JURKAT Cell Lysate
