More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| methyl-CpG binding domain protein 2 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human MBD2 recombinant protein (Position: W159-A411). Human MBD2 shares 98% amino acid (aa) sequence identity with mouse MBD2. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MBD2 |
Protein | Methyl-CpG-binding domain protein 2 |
Uniprot ID | Q9UBB5 |
Function | Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters. |
Tissue Specificity | Highly expressed in brain, heart, kidney, stomach, testis and placenta. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Contains 1 MBD (methyl-CpG-binding) domain. |
Aliases | DEMETHYLASE antibody|DKFZp586O0821 antibody|DMTase antibody|MBD 2 antibody|Mbd2 antibody|MBD2_HUMAN antibody|MBD2a antibody|Methyl CpG binding domain protein 2 antibody|Methyl CpG binding protein MBD2 antibody|Methyl-CpG-binding domain protein 2 antibody|Methyl-CpG-binding protein MBD2 antibody|NY CO 41 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- MBD2 antibody, ASA-B1226, Western blotting
All lanes: Anti MBD2 (ASA-B1226) at 0.5ug/m
lWB: Recombinant Human MBD2 Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
All lanes: Anti MBD2 (ASA-B1226) at 0.5ug/m
lWB: Recombinant Human MBD2 Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
Anti- MBD2 antibody, ASA-B1226, Western blotting
All lanes: Anti MBD2 (ASA-B1226) at 0.5ug/ml
Lane 1: SGC Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: K562 Whole Cell Lysate at 40ug
Predicted bind size: 47KD
Observed bind size: 47KD
All lanes: Anti MBD2 (ASA-B1226) at 0.5ug/ml
Lane 1: SGC Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: K562 Whole Cell Lysate at 40ug
Predicted bind size: 47KD
Observed bind size: 47KD
