More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| methyl-CpG binding domain protein 1 | Polyclonal | IgG | Rabbit | Human, Mouse | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human MBD1(366-381aa NQKRQKCRWRQCLQFA), identical to the related mouse sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MBD1 |
Protein | Methyl-CpG-binding domain protein 1 |
Uniprot ID | Q9UIS9 |
Function | Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters. |
Tissue Specificity | Widely expressed. |
Sub-cellular localization | Nucleus . Nucleus matrix . Nucleus speckle. Chromosome. Note: Colocalizes with the Ten-1 ICD form of TENM1 in foci associated with the nuclear matrix (By similarity). Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes. |
Sequence Similarities | Contains 3 CXXC-type zinc fingers. |
Aliases | CXXC 3 antibody|CXXC-type zinc finger protein 3 antibody|CXXC3 antibody|MBD 1 antibody|MBD1 antibody|MBD1_HUMAN antibody|MECP1 COMPLEX antibody|Methyl CpG binding domain protein 1 antibody|Methyl CpG binding domain protein 1 isoform PCM1 antibody|Methyl CpG binding protein MBD1 antibody|Methyl CpG binding protein splice variant 1 antibody|Methyl CpG binding protein splice variant 2 antibody|Methyl CpG binding protein splice variant 3 antibody|Methyl CpG binding protein splice variant 4 antibody|Methyl-CpG-binding domain protein 1 antibody|Methyl-CpG-binding protein MBD1 antibody|PCM 1 antibody|PCM1 antibody|Protein containing methyl CpG binding domain 1 antibody|Protein containing methyl-CpG-binding domain 1 antibody|Regulator of fibroblast growth factor 2(FGF 2) transcription antibody|RFT antibody|The regulator of fibroblast growth factor 2(FGF 2) transcription antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MBD1 antibody, ASA-B1225, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human MBD1, 38.2KD(162aa tag+ R283-A463)
Lane 1: Recombinant Human MBD1 Protein 10ng
Lane 2: Recombinant Human MBD1 Protein 5ng
Recombinant Protein Detection Source: E.coli derived -recombinant Human MBD1, 38.2KD(162aa tag+ R283-A463)
Lane 1: Recombinant Human MBD1 Protein 10ng
Lane 2: Recombinant Human MBD1 Protein 5ng
