More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| MYC associated factor X | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human MAX(32-48aa ERKRRDHIKDSFHSLRD), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MAX |
Protein | Protein max |
Uniprot ID | P61244 |
Function | Transcription regulator. Forms a sequence-specific DNA- binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. |
Tissue Specificity | High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle. |
Sub-cellular localization | Nucleus. Cell projection, dendrite . |
Sequence Similarities | Belongs to the MAX family. |
Aliases | bHLHd4 antibody|bHLHd5 antibody|bHLHd6 antibody|bHLHd7 antibody|bHLHd8 antibody|Helix loop helix zipper protein antibody|MAX protein antibody|MGC10775 antibody|MGC11225 antibody|MGC18164 antibody|MGC34679 antibody|MGC36767 antibody|Myc associated factor X antibody|Myc binding novel HLH/LZ protein antibody|Orf 1 antibody|Orf1 antibody|Protein max antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MAX antibody, ASA-B1224, Western blotting
All lanes: Anti MAX (ASA-B1224) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 29KD
Observed bind size: 29KD
All lanes: Anti MAX (ASA-B1224) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 29KD
Observed bind size: 29KD
