More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| matrilin 3 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Matrilin 3(466-486aa NTKLDDILEKLKINEYGQIHR). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MATN3 |
Protein | Matrilin-3 |
Uniprot ID | O15232 |
Function | Major component of the extracellular matrix of cartilage and may play a role in the formation of extracellular filamentous networks. |
Tissue Specificity | Expressed only in cartilaginous tissues, such as vertebrae, ribs and shoulders. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Contains 4 EGF-like domains. |
Aliases | AV009181 antibody|DIPOA antibody|EDM5 antibody|HOA antibody|MATN3 antibody|MATN3_HUMAN antibody|Matrilin 3 antibody|Matrilin-3 antibody|OADIP antibody|OS2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Matrilin 3 antibody, ASA-B1223, Western blotting
Lane 1: 293T Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: A549 Cell Lysate
Lane 1: 293T Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: A549 Cell Lysate
