More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human MAFA(136-167aa EDAVEALIGSGHHGAHHGAHHPAAAAAYEAFR), identical to the related mouse sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MAFA |
Protein | Transcription factor MafA |
Uniprot ID | Q8NHW3 |
Function | Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the bZIP family. Maf subfamily. |
Aliases | HMafA antibody|Mafa antibody|MAFA_HUMAN antibody|Pancreatic beta cell specific transcriptional activator antibody|Pancreatic beta-cell-specific transcriptional activator antibody|RIPE3b1 antibody|Transcription factor MafA antibody|Transcription factor RIPE3b1 antibody|v maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian) antibody|v maf musculoaponeurotic fibrosarcoma oncogene homolog A antibody|V-maf musculoaponeurotic fibrosarcoma oncogene homolog A antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- MAFA antibody, ASA-B1208, Western blotting
All lanes: Anti MAFA (ASA-B1208) at 0.5ug/ml
WB: Rat Cardiac Muscle Tissue Lysate at 50ug
Predicted bind size: 37KD
Observed bind size: 37KD
All lanes: Anti MAFA (ASA-B1208) at 0.5ug/ml
WB: Rat Cardiac Muscle Tissue Lysate at 50ug
Predicted bind size: 37KD
Observed bind size: 37KD
