More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| MAD2 mitotic arrest deficient-like 1 (yeast) | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Mad2L1 recombinant protein (Position: A2-D205). Human Mad2L1 shares 94% amino acid (aa) sequence identity with mouse Mad2L1. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MAD2L1 |
Protein | Mitotic spindle assembly checkpoint protein MAD2A |
Uniprot ID | Q13257 |
Function | Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore- spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. Chromosome, centromere, kinetochore. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Note: Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD. |
Sequence Similarities | Belongs to the MAD2 family. |
Aliases | Homolog of MAD2 S cerevisiae antibody|HsMAD 2 antibody|HsMAD2 antibody|Human mitotic feedback control protein Madp2 antibody|MAD 2 antibody|MAD 2A antibody|MAD2 antibody|MAD2 like 1 antibody|MAD2 like protein 1 antibody|MAD2 mitotic arrest deficient like 1 antibody|MAD2-like protein 1 antibody|MAD2A antibody|Mad2l1 antibody|MD2L1_HUMAN antibody|Mitotic arrest deficient 2-like protein 1 antibody|Mitotic arrest deficient like 1 antibody|Mitotic arrest deficient yeast homolog antibody|Mitotic arrest deficient yeast homolog like 1 antibody|Mitotic spindle assembly checkpoint protein antibody|Mitotic spindle assembly checkpoint protein MAD2A antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Mad2L1 antibody, ASA-B1205, Western blotting
All lanes: Anti Mad2L1 (ASA-B1205) at 0.5ug/ml
Lane 1: 293T Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
All lanes: Anti Mad2L1 (ASA-B1205) at 0.5ug/ml
Lane 1: 293T Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
