More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| leukocyte receptor tyrosine kinase | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human LTK(850-864aa RGLQPQNLWNPTYRS). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | LTK |
Protein | Leukocyte tyrosine kinase receptor |
Uniprot ID | P29376 |
Function | Orphan receptor with a tyrosine-protein kinase activity. The exact function of this protein is not known. Studies with chimeric proteins (replacing its extracellular region with that of several known growth factor receptors, such as EGFR and CSFIR) demonstrate its ability to promote growth and specifically neurite outgrowth, and cell survival. Signaling appears to involve the PI3 kinase pathway. Involved in regulation of the secretory pathway involving endoplasmic reticulum (ER) export sites (ERESs) and ER to Golgi transport. |
Tissue Specificity | Expressed in non-hematopoietic cell lines and T- and B-cell lines. |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. |
Sequence Similarities | Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. |
Aliases | ALK antibody|Anaplastic lymphoma kinase antibody|Leukocyte receptor tyrosine kinase antibody|Leukocyte tyrosine kinase antibody|Leukocyte tyrosine kinase receptor antibody|Ltk antibody|LTK_HUMAN antibody|Protein tyrosine kinase 1 antibody|TYK 1 antibody|TYK1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-LTK antibody, ASA-B1194, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human LTK,33.1KD(162aa tag+ N729-S864)
Lane 1: Recombinant Human LTK Protein 10ng
Lane 2: Recombinant Human LTK Protein 5ng
Lane 3: Recombinant Human LTK Protein 2.5ng
Lane 4: Recombinant Human LTK Protein 1.25ng
Recombinant Protein Detection Source: E.coli derived -recombinant Human LTK,33.1KD(162aa tag+ N729-S864)
Lane 1: Recombinant Human LTK Protein 10ng
Lane 2: Recombinant Human LTK Protein 5ng
Lane 3: Recombinant Human LTK Protein 2.5ng
Lane 4: Recombinant Human LTK Protein 1.25ng
