More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| low density lipoprotein receptor-related protein 5 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human LRP5(1201-1215aa IHAVEEVSLEEFSAH), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | LRP5 |
Protein | Low-density lipoprotein receptor-related protein 5 |
Uniprot ID | O75197 |
Function | Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor- ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3- mediated phosphorylation and destruction of beta-catenin. Appears be required for postnatal control of vascular regression in the eye. Required for posterior patterning of the epiblast during gastrulation. |
Tissue Specificity | Widely expressed, with the highest level of expression in the liver and in aorta. |
Sub-cellular localization | Membrane; Single-pass type I membrane protein. Endoplasmic reticulum . Note: Chaperoned to the plasma membrane by MESD. |
Sequence Similarities | Belongs to the LDLR family. |
Aliases | BMND1 antibody|EVR1 antibody|EVR4 antibody|HBM antibody||HGNC:6697 antibody|Low density lipoprotein receptor related protein 5 antibody|Low density lipoprotein receptor related protein 7 antibody|Low-density lipoprotein receptor-related protein 5 antibody|LR3 antibody|LRP-5 antibody|Lrp5 antibody|LRP7 antibody|OPPG antibody|OPS antibody|OPTA1 antibody|Osteoporosis pseudoglioma syndrome antibody|VBCH2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-LRP5 antibody, ASA-B1191, All Western blotting
All lanes: Anti-LRP5(ASA-B1191) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Mouse Liver Tissue Lysate at 40ug
Lane 3: NIH Whole Cell Lysate at 40ug
Predicted bind size: 179KD
Observed bind size: 179KD
All lanes: Anti-LRP5(ASA-B1191) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Mouse Liver Tissue Lysate at 40ug
Lane 3: NIH Whole Cell Lysate at 40ug
Predicted bind size: 179KD
Observed bind size: 179KD
