More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| LIM domain containing preferred translocation partner in lipoma | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human LPP(291-307aa PNQGRYYEGYYAAGPGY). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | LPP |
Protein | Lipoma-preferred partner |
Uniprot ID | Q93052 |
Function | May play a structural role at sites of cell adhesion in maintaining cell shape and motility. In addition to these structural functions, it may also be implicated in signaling events and activation of gene transcription. May be involved in signal transduction from cell adhesion sites to the nucleus allowing successful integration of signals arising from soluble factors and cell-cell adhesion sites. Also suggested to serve as a scaffold protein upon which distinct protein complexes are assembled in the cytoplasm and in the nucleus. |
Tissue Specificity | Expressed in a wide variety of tissues but no or very low expression in brain and peripheral leukocytes. |
Sub-cellular localization | Nucleus. Cytoplasm. Cell junction. Cell membrane. Note: Found in the nucleus, in the cytoplasm and at cell adhesion sites. Shuttles between the cytoplasm and the nucleus. It has been found in sites of cell adhesion such as cell-to-cell contact and focal adhesion which are membrane attachment sites of cells to the extracellular matrix. Mainly nuclear when fused with HMGA2/HMGIC and KMT2A/MLL1. |
Sequence Similarities | Belongs to the zyxin/ajuba family. |
Aliases | DKFZp779O0231 antibody|FLJ30652 antibody|FLJ41512 antibody|LIM domain containing preferred translocation partner in lipoma antibody|LIM domain-containing preferred translocation partner in lipoma antibody|LIM protein antibody|Lipoma preferred partner antibody|Lipoma-preferred partner antibody|lpp antibody|LPP_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-LPP antibody, ASA-B1189, Western blotting
Lane 1: SMMC Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SW620 Cell Lysate
Lane 4: A549 Cell Lysate
Lane 5: SKOV Cell Lysate
Lane 6: MCF-7 Cell Lysate
Lane 1: SMMC Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SW620 Cell Lysate
Lane 4: A549 Cell Lysate
Lane 5: SKOV Cell Lysate
Lane 6: MCF-7 Cell Lysate
