More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| oxidized low density lipoprotein(lectin-like) receptor 1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human LOX 1(1-16aa MTFDDLKIQTVKDQPD). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | OLR1 |
Protein | Oxidized low-density lipoprotein receptor 1(Ox-LDL receptor 1) |
Uniprot ID | P78380 |
Function | Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro- oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro- atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram- positive bacteria. |
Tissue Specificity | Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level. |
Sub-cellular localization | Cell membrane; Lipid-anchor. Cell membrane; Single-pass type II membrane protein. Membrane raft. Secreted. Note: A secreted form also exists. Localization to membrane rafts requires palmitoylation. |
Sequence Similarities | Contains 1 C-type lectin domain. |
Aliases | C-type lectin domain family 8 member A antibody|CLEC8A antibody|hLOX 1 antibody|hLOX-1 antibody|Lectin like oxidized LDL receptor 1 antibody|Lectin like oxLDL receptor 1 antibody|Lectin type oxidized LDL receptor 1 antibody|Lectin-like oxidized LDL receptor 1 antibody|Lectin-like oxLDL receptor 1 antibody|Lectin-type oxidized LDL receptor 1 antibody|LOX-1 antibody|LOXIN antibody|Olr1 antibody|OLR1_HUMAN antibody|Ox LDL receptor 1 antibody|Ox-LDL receptor 1 antibody|Oxidised low density lipoprotein(lectin like) receptor 1 antibody|Oxidized low density lipoprotein receptor 1 antibody|Oxidized low density lipoprotein receptor 1 soluble form antibody|Oxidized low-density lipoprotein receptor 1 antibody|SCARE1 antibody|Scavenger receptor class E, member 1 antibody|SLOX1 antibody|soluble form antibody|SR-EI antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-LOX 1 antibody, ASA-B1187, Western blotting
All lanes: Anti LOX 1 (ASA-B1187) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: U937 Whole Cell Lysate at 40ug
Lane 5: K562 Whole Cell Lysate at 40ug
Predicted bind size:31 KD
Observed bind size: 31KD
All lanes: Anti LOX 1 (ASA-B1187) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: U937 Whole Cell Lysate at 40ug
Lane 5: K562 Whole Cell Lysate at 40ug
Predicted bind size:31 KD
Observed bind size: 31KD
