More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| lipoprotein lipase | Polyclonal | IgG | Rabbit | Bovine, Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Lipoprotein lipase(448-170aa EKVSHLQKGKAPAVFVKCHDKSL), different from the related mouse and rat sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | LPL |
Protein | Lipoprotein lipase(LPL) |
Uniprot ID | P06858 |
Function | The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium (By similarity). |
Tissue Specificity | |
Sub-cellular localization | Cell membrane ; Lipid-anchor, GPI-anchor . Secreted . Note: Locates to the plasma membrane of microvilli of hepatocytes with triacyl- glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles (By similarity). |
Sequence Similarities | Belongs to the AB hydrolase superfamily. Lipase family. |
Aliases | EC 3.1.1 antibody|EC 3.1.1.34 antibody|HDLCQ11 antibody|LIPD antibody|LIPL_HUMAN antibody|Lipoprotein lipase antibody|LPL antibody|LPL protein antibody|MGC137861 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Bovine Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Lipoprotein lipase antibody, ASA-B1176, Western blotting
All lanes: Anti -Lipoprotein lipase (ASA-B1176) at 0.5ug/ml
Lane 1: Milk
Lane 2: M CF-7 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 53KD
Observed bind size: 53KD,70KD
All lanes: Anti -Lipoprotein lipase (ASA-B1176) at 0.5ug/ml
Lane 1: Milk
Lane 2: M CF-7 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 53KD
Observed bind size: 53KD,70KD
