More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| leukemia inhibitory factor | Polyclonal | IgG | Rabbit | Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse LIF(175-194aa DKEAFQRKKLGCQLLGTYKQ), identical to the related rat sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | LIF |
Protein | Leukemia inhibitory factor(LIF) |
Uniprot ID | P09056 |
Function | LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | |
Aliases | CDF antibody|Cholinergic Differentiation Factor antibody|D factor antibody|DIA antibody|Differentiation inducing factor antibody|differentiation inhibitory activity antibody|Differentiation stimulating factor antibody|Differentiation-stimulating factor antibody|Emfilermin antibody|Hepatocyte stimulating factor III antibody|HILDA antibody|Human interleukin in DA cells antibody|Leukemia inhibitory factor antibody|LIF antibody|Lif antibody|LIF_HUMAN antibody|Melanoma derived LPL inhibitor antibody|Melanoma-derived LPL inhibitor antibody|MLPLI antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-LIF antibody, ASA-B1168, Western blotting
All lanes: Anti LIF (ASA-B1168) at 0.5ug/ml
Lane 1: Rat Intestine Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: Rat Thymus Tissue Lysate at 50ug
Lane 5: Mouse Brain Tissue Lysate at 50ug
Lane 6: Mouse Kidney Tissue Lysate at 50ug
Lane 7: Mouse Liver Tissue Lysate at 50ug
Predicted bind size: 22KD
Observed bind size: 40KD
All lanes: Anti LIF (ASA-B1168) at 0.5ug/ml
Lane 1: Rat Intestine Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: Rat Thymus Tissue Lysate at 50ug
Lane 5: Mouse Brain Tissue Lysate at 50ug
Lane 6: Mouse Kidney Tissue Lysate at 50ug
Lane 7: Mouse Liver Tissue Lysate at 50ug
Predicted bind size: 22KD
Observed bind size: 40KD
