More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| lecithin-cholesterol acyltransferase | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human LCAT(300-313aa RDFQRFFADLHFEE), different from the related rat sequence by two amino acids, and from the mouse sequence by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | LCAT |
Protein | Phosphatidylcholine-sterol acyltransferase |
Uniprot ID | P04180 |
Function | Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms. |
Tissue Specificity | Expressed mainly in brain, liver and testes. Secreted into plasma and cerebral spinal fluid. Expressed in Hep- G2 cell line. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the AB hydrolase superfamily. Lipase family. |
Aliases | LCAT antibody|LCAT_HUMAN antibody|Lecithin cholesterol acyltransferase antibody|Lecithin-cholesterol acyltransferase antibody|Phosphatidylcholine sterol acyltransferase antibody|Phosphatidylcholine-sterol acyltransferase antibody|Phospholipid cholesterol acyltransferase antibody|Phospholipid-cholesterol acyltransferase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-LCAT antibody, ASA-B1160, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: U87 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: SMMC Cell Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: U87 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: SMMC Cell Lysate
