More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| lysosomal-associated membrane protein 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human LAMP1(403-417aa YLVGRKRSHAGYQTI), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | LAMP1 |
Protein | Lysosome-associated membrane glycoprotein 1(LAMP-1) |
Uniprot ID | P11279 |
Function | Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Late endosome. Note: This protein shuttles between lysosomes, endosomes, and the plasma membrane. Colocalizes with OSBPL1A at the late endosome. |
Sequence Similarities | |
Aliases | CD107 antigen like family member A antibody|CD107 antigen-like family member A antibody|CD107a antibody|CD107a antigen antibody|LAMP 1 antibody|LAMP-1 antibody|LAMP1 antibody|LAMP1_HUMAN antibody|LAMPA antibody|LGP120 antibody|lgpA antibody|Lysosomal membrane glycoprotein 120KD antibody|Lysosomal Associated Membrane Protein 1 antibody|Lysosome associated membrane glycoprotein 1 antibody|Lysosome-associated membrane glycoprotein 1 antibody|Lysosome-associated membrane protein 1 antibody|OTTHUMP00000040663 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- LAMP1antibody, ASA-B1156, Western blotting
All lanes: Anti LAMP1 (ASA-B1156) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: A549 Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 80KD
All lanes: Anti LAMP1 (ASA-B1156) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: A549 Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 80KD
