More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| laminin, alpha 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Laminin 2 alpha(152-170aa WILERSLDDVEYKPWQYHA), identical to the related mouse sequence, and different from the related rat sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | LAMA2 |
Protein | Laminin subunit alpha-2 |
Uniprot ID | P24043 |
Function | Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. |
Tissue Specificity | Placenta, striated muscle, peripheral nerve, cardiac muscle, pancreas, lung, spleen, kidney, adrenal gland, skin, testis, meninges, choroid plexus, and some other regions of the brain; not in liver, thymus and bone. |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix, basement membrane. Note: Major component. |
Sequence Similarities | Contains 17 laminin EGF-like domains. |
Aliases | LAMA 2 antibody|LAMA2 antibody|LAMA2_HUMAN antibody|Laminin alpha 2(merosin congenital muscular dystrophy) antibody|Laminin alpha 2 antibody|Laminin alpha 2 chain antibody|Laminin alpha 2 subunit antibody|Laminin M antibody|Laminin M chain antibody|Laminin subunit alpha-2 antibody|Laminin-12 subunit alpha antibody|Laminin-2 subunit alpha antibody|Laminin-4 subunit alpha antibody|LAMM antibody|Merosin heavy chain antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Laminin 2 alpha antibody, ASA-B1152, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: A549 Cell Lysate
Lane 3: PANC Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: A549 Cell Lysate
Lane 3: PANC Cell Lysate
