More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| potassium voltage-gated channel, shaker-related subfamily, member 6 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Kv1.6(515-529aa TPHRAYAEKRMLTEV), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | KCNA6 |
Protein | Potassium voltage-gated channel subfamily A member 6 |
Uniprot ID | P17658 |
Function | Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient (PubMed:2347305, PubMed:14575698). The channel alternates between opened and closed conformations in response to the voltage difference across the membrane (PubMed:2347305, PubMed:14575698). Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA6, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (By similarity). Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation (By similarity). Homotetrameric channels display rapid activation and slow inactivation (PubMed:2347305). |
Tissue Specificity | |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | |
Aliases | HBK 2 antibody|HBK2 antibody|Human brain potassium channel 2 antibody|KCNA 6 antibody|Kcna6 antibody|Potassium voltage gated channel shaker related subfamily member 6 antibody|Potassium voltage gated channel subfamily A member 6 antibody|Potassium voltage-gated channel subfamily A member 6 antibody|Voltage gated potassium channel protein Kv1.6 antibody|Voltage gated potassium channel subunit Kv1.6 antibody|Voltage-gated potassium channel HBK2 antibody|Voltage-gated potassium channel subunit Kv1.6 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Kv1.6 antibody, ASA-B1142, All Western blotting
All lanes: Anti-KCNA6(ASA-B1142) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Lane 2: Mouse Brain Tissue Lysate at 40ug
Predicted bind size: 59KD
Observed bind size: 70KD
All lanes: Anti-KCNA6(ASA-B1142) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Lane 2: Mouse Brain Tissue Lysate at 40ug
Predicted bind size: 59KD
Observed bind size: 70KD
