KRAS Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human KRAS(164-178aa/b RKHKEKMSKDGKKKK), identical to the related mouse and rat sequences.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	KRAS
Protein	GTPase Kras
Uniprot ID	P01116
Function	Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838).
Tissue Specificity
Sub-cellular localization	Cell membrane.
Sequence Similarities	Belongs to the small GTPase superfamily. Ras family.
Aliases	c Kirsten ras protein antibody|c-K-ras antibody|c-Ki-ras antibody|Cellular c Ki ras2 proto oncogene antibody|cK Ras antibody|GTPase KRas antibody|K RAS p21 protein antibody|K RAS2A antibody|K RAS2B antibody|K RAS4A antibody|K RAS4B antibody|K-Ras 2 antibody|KI RAS antibody|Ki-Ras antibody|KIRSTEN MURINE SARCOMA VIRUS 2 antibody|Kirsten rat sarcoma 2 viral(v Ki ras2) oncogene homolog antibody|KRAS antibody|KRAS1 antibody|KRAS2 antibody|N-terminally processed antibody|NS antibody|NS3 antibody|Oncogene KRAS2 antibody|p21ras antibody|PR310 cK ras oncogene antibody|RASK_HUMAN antibody|RASK2 antibody|Transforming protein p21 antibody|v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Mouse, Rat	AssaySolutio's ECL kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information