More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| myeloid/lymphoid or mixed-lineage leukemia(trithorax homolog, Drosophila) | Polyclonal | IgG | Rabbit | Human, Mouse | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human KMT2A(1-15aa MAHSCRWRFPARPGT), identical to the related mouse sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MLL |
Protein | Histone-lysine N-methyltransferase MLL |
Uniprot ID | Q03164 |
Function | Histone methyltransferase that plays an essential role in early development and hematopoiesis. Catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac). In the MLL1/MLL complex, it specifically mediates H3K4me, a specific tag for epigenetic transcriptional activation. Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity. Has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', while it has higher activity toward H3 acetylated on 'Lys-9'. Required for transcriptional activation of HOXA9. Promotes PPP1R15A-induced apoptosis. Plays a critical role in the control of circadian gene expression and is essential for the transcriptional activation mediated by the CLOCK-ARNTL/BMAL1 heterodimer. Establishes a permissive chromatin state for circadian transcription by mediating a rhythmic methylation of 'Lys-4' of histone H3 (H3K4me) and this histone modification directs the circadian acetylation at H3K9 and H3K14 allowing the recruitment of CLOCK-ARNTL/BMAL1 to chromatin (By similarity). |
Tissue Specificity | Heart, lung, brain and T- and B-lymphocytes. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX/MLL subfamily. |
Aliases | Acute lymphocytic leukemia 1 antibody|ALL 1 antibody|ALL-1 antibody|ALL1 antibody|C-terminal cleavage product of 180 kDa antibody|CXXC 7 antibody|CXXC-type zinc finger protein 7 antibody|CXXC7 antibody|HRX antibody|HTRX 1 antibody|HTRX1 antibody|KMT2A antibody|Lysine N-methyltransferase 2A antibody|MLL 1A antibody|Mll antibody|MLL cleavage product C180 antibody|MLL1 antibody|MLL1_HUMAN antibody|MLL1A antibody|Myeloid/lymphoid or mixed lineage leukemia antibody|N-terminal cleavage product of 320 kDa antibody|p180 antibody|p320 antibody|Trithorax homolog antibody|Trithorax homolog Drosophila antibody|Trithorax like protein antibody|Trithorax-like protein antibody|TRX 1 antibody|TRX1 antibody|Zinc finger protein HRX antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- MLL antibody, ASA-B1132, Western blotting
All lanes: Anti MLL (ASA-B1132) at 0.5ug/ml
WB: 293T Whole Cell Lysate at 40ug
Predicted bind size: 320KD
Observed bind size: 320KD
All lanes: Anti MLL (ASA-B1132) at 0.5ug/ml
WB: 293T Whole Cell Lysate at 40ug
Predicted bind size: 320KD
Observed bind size: 320KD
