More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| Kruppel-like factor 6 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human KLF6 recombinant protein (Position: E38-N205). Human KLF6 shares 92% and 90% amino acid (aa) sequences identity with mouse and rat KLF6, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | KLF6 |
Protein | Krueppel-like factor 6 |
Uniprot ID | Q99612 |
Function | Transcriptional activator (By similarity). Binds a GC box motif. Could play a role in B-cell growth and development. |
Tissue Specificity | Highly expressed in placenta followed by spleen, thymus, prostate, testis, small intestine and colon. Weakly expressed in pancreas, lung, liver, heart and skeletal muscle. Also expressed in fetal brain, spleen and thymus. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the krueppel C2H2-type zinc-finger protein family. |
Aliases | B cell derived protein 1 antibody|B cell-derived 1 antibody|B-cell-derived protein 1 antibody|BCD1 antibody|CBA1 antibody|COPEB antibody|Core promoter element-binding protein antibody|CPBP antibody|GBF antibody|GC rich binding factor antibody|GC rich sites binding factor GBF antibody|GC-rich sites-binding factor GBF antibody|Klf6 antibody|KLF6_HUMAN antibody|Krueppel-like factor 6 antibody|Krueppel-like factor 6 isoform C antibody|Kruppel like zinc finger protein Zf9 antibody|PAC1 antibody|Proto-oncogene BCD1 antibody|Protooncogene B cell derived 1 antibody|ST12 antibody|Suppression of tumorigenicity 12 (prostate) antibody|Suppressor of tumorigenicity 12 protein antibody|Transcription factor Zf9 antibody|Zf9 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- KLF6 antibody, ASA-B1130, Western blotting
All lanes: Anti KLF6 (ASA-B1130) at 0.5ug/ml
WB: Recombinant Human KLF6 Protein 0.5ng
Predicted bind size: 36KD
Observed bind size: 36KD
All lanes: Anti KLF6 (ASA-B1130) at 0.5ug/ml
WB: Recombinant Human KLF6 Protein 0.5ng
Predicted bind size: 36KD
Observed bind size: 36KD
Anti- KLF6 antibody, ASA-B1130, Western blotting
All lanes: Anti KLF6 (ASA-B1130) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: Rat Testis Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: HEPG2 Whole Cell Lysate at 40ug
Lane 5: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 32KD
Observed bind size: 37KD
All lanes: Anti KLF6 (ASA-B1130) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: Rat Testis Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: HEPG2 Whole Cell Lysate at 40ug
Lane 5: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 32KD
Observed bind size: 37KD
