More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| Kruppel-like factor 4(gut) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human KLF4(93-108aa RRETEEFNDLLDLDFI), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | KLF4 |
Protein | Krueppel-like factor 4 |
Uniprot ID | O43474 |
Function | Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the krueppel C2H2-type zinc-finger protein family. |
Aliases | Endothelial Kruppel like zinc finger protein antibody|Epithelial zinc finger protein EZF antibody|EZF antibody|GKLF antibody|Gut-enriched krueppel-like factor antibody|KLF antibody|KLF4 antibody|KLF4_HUMAN antibody|Krueppel-like factor 4 antibody|Kruppel like factor 4(Epithelial zinc finger protein EZF)(Gut enriched Krueppel like factor) antibody|Kruppel like factor 4(gut) antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-KLF4 antibody, ASA-B1128, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: A549 Cell Lysate
Lane 3: U20S Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: A549 Cell Lysate
Lane 3: U20S Cell Lysate
