More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| kininogen 1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Kininogen 1 recombinant protein (Position: Q19-N210). Human Kininogen 1 shares 63% and 66% amino acid (aa) sequence identity with mouse and rat Kininogen 1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | KNG1 |
Protein | Kininogen-1 |
Uniprot ID | P01042 |
Function | (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin- induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW- kininogen is in contrast to HMW-kininogen not involved in blood clotting. |
Tissue Specificity | Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors. |
Sub-cellular localization | Secreted, extracellular space. |
Sequence Similarities | Contains 3 cystatin kininogen-type domains. |
Aliases | Alpha-2-thiol proteinase inhibitor antibody|BDK antibody|BK antibody|Bradykinin antibody|Fitzgerald factor antibody|High molecular weight kininogen antibody|HMWK antibody|Ile-Ser-Bradykinin antibody|Kallidin I antibody|Kallidin II antibody|Kininogen antibody|Kininogen1|KNG antibody|KNG1 antibody|KNG1_HUMAN antibody|Low molecular weight growth-promoting factor antibody|Williams-Fitzgerald-Flaujeac factor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Kininogen1 antibody, ASA-B1124, Western blotting
All lanes: Anti Kininogen1(ASA-B1124) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: SKOV Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 72KD
Observed bind size: 72KD
All lanes: Anti Kininogen1(ASA-B1124) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: SKOV Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 72KD
Observed bind size: 72KD
