More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| KIN, antigenic determinant of recA protein homolog(mouse) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human KIN(48-64aa ESHQRQLLLASENPQQF), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | KIN |
Protein | DNA/RNA-binding protein KIN17 |
Uniprot ID | O60870 |
Function | Involved in DNA replication and the cellular response to DNA damage. May participate in DNA replication factories and create a bridge between DNA replication and repair mediated by high molecular weight complexes. May play a role in illegitimate recombination and regulation of gene expression. May participate in mRNA processing. Binds, in vitro, to double-stranded DNA. Also shown to bind preferentially to curved DNA in vitro and in vivo (By similarity). Binds via its C-terminal domain to RNA in vitro. |
Tissue Specificity | Ubiquitously expressed in all tissues examined, with highest levels in skeletal muscle, heart and testis. Differentially expressed in non-tumorigenic and tumorigenic cell lines. Highly expressed in proliferating epithelial keratinocyte cells in vitro (at protein level). |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the KIN17 family. |
Aliases | BTCD antibody|KIN17 antibody|Binding to curved DNA antibody|antigenic determinant of recA protein(mouse) homolog antibody|KIN, antigenic determinant of recA protein homolog antibody|ANTIGENIC DETERMINANT OF RECA, MOUSE, HOMOLOG OF antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-KIN antibody, ASA-B1122, Western blotting
Lane 1: Rat Skeletal Muscle Tissue Lysate
Lane 2: Human Placenta Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Lane 1: Rat Skeletal Muscle Tissue Lysate
Lane 2: Human Placenta Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
