More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| antigen identified by monoclonal antibody Ki-67 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Ki67(3234-3256aa KKAEDNVCVKKIRTRSHRDSEDI). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | MKI67 |
Protein | Antigen KI-67 |
Uniprot ID | P46013 |
Function | Thought to be required for maintaining cell proliferation. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Contains 1 FHA domain. |
Aliases | Antigen identified by monoclonal antibody Ki 67 antibody|Antigen KI-67 antibody|Antigen KI67 antibody|KI67_HUMAN antibody|KIA antibody|MKI67 antibody|Proliferation related Ki 67 antigen antibody|RP11-380J17.2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Ki67 antibody, ASA-B1118, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human Ki67 50.3KD(162aa tag+ K2967-I3256)
Lane 1: Recombinant Human Ki67 Protein 10ng
Lane 2: Recombinant Human Ki67 Protein 5ng
Lane 3: Recombinant Human Ki67 Protein 2.5ng
Recombinant Protein Detection Source: E.coli derived -recombinant Human Ki67 50.3KD(162aa tag+ K2967-I3256)
Lane 1: Recombinant Human Ki67 Protein 10ng
Lane 2: Recombinant Human Ki67 Protein 5ng
Lane 3: Recombinant Human Ki67 Protein 2.5ng
