More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| hairy and enhancer of split 1,(Drosophila) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
| A synthetic peptide corresponding to a sequence in the middle region of human Hes1(142-155aa HLANCMTQINAMTY), identical to the related mouse and rat sequences. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HES1 |
Protein | Transcription factor HES-1 |
Uniprot ID | Q14469 |
Function | Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity (By similarity). May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Contains 1 bHLH (basic helix-loop-helix) domain. |
Aliases | bHLHb39 antibody|C-HAIRY1 antibody|c-hairy1A antibody|Class B basic helix-loop-helix protein 39 antibody|FLJ20408 antibody|Hairy and enhancer of split 1(Drosophila) antibody|Hairy and enhancer of split 1 antibody|Hairy Enhancer of Split 1 antibody|Hairy homolog(Drosophila) antibody|Hairy Homolog antibody|Hairy like antibody|Hairy, Drosophila, homolog of antibody|Hairy-like protein antibody|Hairy/enhancer of split, Drosophila, homolog of, 1 antibody|HAIRY1 antibody|HES-1 antibody|hes1 antibody|Hes1 hairy and enhancer of split 1(Drosophila) antibody|HES1_HUMAN antibody|HHL antibody|HL antibody|HRY antibody|MGC129109 antibody|OTTHUMP00000209031 antibody|RHL antibody|Transcription factor HES 1 antibody|Transcription factor HES-1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human, Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Hes1 antibody, ASA-B0855, Western blotting
WB: Rat Kidney Tissue Lysate
