More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| hepsin | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Hepsin(392-410aa KVSDFREWIFQAIKTHSEA), different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HPN |
Protein | Serine protease hepsin |
Uniprot ID | P05981 |
Function | Plays an essential role in cell growth and maintenance of cell morphology. May mediate the activating cleavage of HGF and MST1/HGFL. Plays a role in the proteolytic processing of ACE2. |
Tissue Specificity | Present in most tissues, with the highest level in liver. |
Sub-cellular localization | Membrane; Single-pass type II membrane protein. |
Sequence Similarities | Belongs to the peptidase S1 family. |
Aliases | EC 3.4.21. antibody|HEPS antibody|HEPS_HUMAN antibody|Hepsin antibody|HPN antibody|Serine protease hepsin antibody|Serine protease hepsin catalytic chain antibody|Serine protease hepsin non catalytic chain antibody|TMPRSS 1 antibody|TMPRSS1 antibody|Transmembrane protease serine 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Hepsin antibody, ASA-B0854, Western blotting
Lane 1: 293T Cell Lysate
Lane 2: NRK Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: PANC Cell Lysate
Lane 5: MFC-7 Cell Lysate
Lane 1: 293T Cell Lysate
Lane 2: NRK Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: PANC Cell Lysate
Lane 5: MFC-7 Cell Lysate
