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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| DnaJ(Hsp40) homolog, subfamily A, member 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human HDJ2(369-384aa NQERRRHYNGEAYEDD), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | DNAJA1 |
Protein | DnaJ homolog subfamily A member 1 |
Uniprot ID | P31689 |
Function | Co-chaperone for HSPA8/Hsc70 (PubMed:10816573). Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro) (PubMed:24318877). Plays a role in protein transport into mitochondria via its role as co- chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis (PubMed:14752510). Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV (PubMed:24512202). |
Tissue Specificity | Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver. |
Sub-cellular localization | Membrane. |
Sequence Similarities | Contains 1 CR-type zinc finger. |
Aliases | DJ 2 antibody|DJ2 antibody|DjA1 antibody|DnaJ(Hsp40) homolog, subfamily A, member 1 antibody|DnaJ homolog subfamily A member 1 antibody|DnaJ protein homolog 2 antibody|DNAJ2 antibody|Dnaja1 antibody|DNJA1_HUMAN antibody|hDJ 2 antibody|HDJ-2 antibody|HDJ2 antibody|Heat shock 40 kDa protein 4 antibody|heat shock protein DNAJ like 2 antibody|Heat shock protein J2 antibody|HSDJ antibody|HSJ-2 antibody|HSJ2 antibody|HSPF4 antibody|Human DnaJ protein 2 antibody|NEDD7 antibody|Neural precursor cell expressed developmentally down regulated 7 antibody|OTTHUMP00000021193 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HDJ2 antibody, ASA-B0849, Western blotting
All lanes: Anti HDJ2 (ASA-B0849) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: Mouse Brain Tissue Lysate at 50ug
Lane 4: Mouse Lung Tissue Lysate at 50ug
Lane 5: U87 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: A431 Whole Cell Lysate at 40ug
Lane 9: HT1080 Whole Cell Lysate at 40ug
Pre
All lanes: Anti HDJ2 (ASA-B0849) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: Mouse Brain Tissue Lysate at 50ug
Lane 4: Mouse Lung Tissue Lysate at 50ug
Lane 5: U87 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: A431 Whole Cell Lysate at 40ug
Lane 9: HT1080 Whole Cell Lysate at 40ug
Pre
