More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| hyperpolarization activated cyclic nucleotide-gated potassium channel 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human HCN1 recombinant protein (Position: E618-L890). Human HCN1 shares 82% amino acid (aa) sequence identity with both mouse and rat HCN1. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HCN1 |
Protein | Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 |
Uniprot ID | O60741 |
Function | Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). May mediate responses to sour stimuli. |
Tissue Specificity | Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas. |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | Belongs to the potassium channel HCN family. |
Aliases | BCNG-1 antibody|BCNG1 antibody|Brain cyclic nucleotide gated channel 1 antibody|Brain cyclic nucleotide-gated channel 1 antibody|HAC 2 antibody|HAC2 antibody|HCN1 antibody|HCN1_HUMAN antibody|Hyperpolarization activated cyclic nucleotide gated potassium channel 1 antibody|Potassium channel, voltage-gated, brain, 1 antibody|Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 1 antibody|Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- HCN1 antibody, ASA-B0838, Western blotting
All lanes: Anti HCN1 (ASA-B0838) at 0.5ug/ml
WB: Recombinant Human HCN1 Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
All lanes: Anti HCN1 (ASA-B0838) at 0.5ug/ml
WB: Recombinant Human HCN1 Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
Anti- HCN1 antibody, ASA-B0838, Western blotting
All lanes: Anti HCN1 (ASA-B0838) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: U87 Whole Cell Lysate at 40ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 99KD
Observed bind size: 99KD
All lanes: Anti HCN1 (ASA-B0838) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: U87 Whole Cell Lysate at 40ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 99KD
Observed bind size: 99KD
