More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| heat shock protein 90kDa beta (Grp94), member 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human GRP94 recombinant protein (Position: R43-H221). Human GRP94 shares 99.4% and 98.9% amino acid (aa) sequence identity with mouse and rat GRP94, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HSP90B1 |
Protein | Endoplasmin |
Uniprot ID | P14625 |
Function | |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | |
Aliases | 94 kDa glucose regulated protein antibody|94 kDa glucose-regulated protein antibody|ECGP antibody|Endoplasmin antibody|Endothelial cell (HBMEC) glycoprotein antibody|ENPL_HUMAN antibody|Glucose regulated protein 94 antibody|Glucose regulated protein 94kDa antibody| gp96 antibody|gp96 homolog antibody|GRP 94 antibody|GRP-94 antibody|Heat shock protein 90 kDa beta member 1 antibody|heat shock protein 90kDa beta (Grp94), member 1 antibody|Heat shock protein, 90 kDa, beta, 1 antibody|HSP90B1 antibody|Stress inducible tumor rejection antigen GP96 antibody|TRA1 antibody|tumor rejection antigen (gp96) 1 antibody|Tumor rejection antigen 1 antibody|Tumor rejection antigen gp96 antibody|Tumor rejection antigen-1 (gp96) antibody |
Application Details
Anti- GRP94 antibody, ASA-B0828, Western blotting
All lanes: Anti GRP94 (ASA-B0828) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: A375 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 92KD
Observed bind size: 92KD
All lanes: Anti GRP94 (ASA-B0828) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: A375 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 92KD
Observed bind size: 92KD
