More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| growth hormone receptor | Polyclonal | IgG | Rabbit | Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of mouse Growth hormone receptor(231-247aa DKEHEVRVRSRQRSFEK), identical to the related rat sequence. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GHR |
Protein | Growth hormone receptor(GH receptor) |
Uniprot ID | P16882 |
Function | Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to, and activates the JAK2/STAT5 pathway (By similarity). |
Tissue Specificity | Expressed in all tissues tested including, liver, heart, adipose tissue, mammary gland, testes, ovary, brain, kidney and muscle. Highest levels in liver. |
Sub-cellular localization | Cell membrane; Single-pass type I membrane protein. Note: On growth hormone binding, GHR is ubiquitinated, internalized, down-regulated and transported into a degradative or non-degradative pathway. |
Sequence Similarities | Belongs to the type I cytokine receptor family. Type 1 subfamily. |
Aliases | GH receptor antibody|GH-binding protein antibody|GHBP antibody|GHBP, included antibody|GHR antibody|GHR_HUMAN antibody|Growth hormone binding protein antibody|Growth hormone receptor antibody|Growth hormone receptor precursor antibody|Growth hormone-binding protein antibody|Growth hormone-binding protein, included antibody|Increased responsiveness to growth hormone, included antibody|Serum binding protein antibody|Serum-binding protein antibody|Somatotropin receptor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-GHR antibody, ASA-B0822, Western blotting
All lanes: Anti GHR (ASA-B0822) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: Rat Intestine Tissue Lysate at 50ug
Lane 5: Mouse Spleen Tissue Lysate at 50ug
Lane 6: Mouse Testis Tissue Lysate at 50ug
Lane 7: Mouse Liver Tissue Lysate at 50ug
Lane 8: Mouse Kidney Tissue Lysate at 50ug
Lane 9: Mouse Intestine Tissue L
All lanes: Anti GHR (ASA-B0822) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: Rat Intestine Tissue Lysate at 50ug
Lane 5: Mouse Spleen Tissue Lysate at 50ug
Lane 6: Mouse Testis Tissue Lysate at 50ug
Lane 7: Mouse Liver Tissue Lysate at 50ug
Lane 8: Mouse Kidney Tissue Lysate at 50ug
Lane 9: Mouse Intestine Tissue L
