More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| chemokine(C-X-C motif) ligand 1(melanoma growth stimulating activity, alpha) | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human GRO alpha(99-107aa KMLNSDKSN). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CXCL1 |
Protein | Growth-regulated alpha protein |
Uniprot ID | P09341 |
Function | Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO- alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the intercrine alpha (chemokine CxC) family. |
Aliases | C-X-C motif chemokine 1 antibody|Chemokine(C X C motif) ligand 1 antibody|Chemokine(C-X-C motif) ligand 1(melanoma growth stimulating activity, alpha) antibody|chemokine(C-X-C motif) ligand 1 antibody|Chemokine ligand 1 antibody|CINC-1 antibody|Cxcl 1 antibody|Cxcl1 antibody|Cytokine-induced neutrophil chemoattractant 1 antibody|Fibroblast secretory protein antibody|Fsp antibody|Gro 1 antibody|Gro A antibody|GRO alpha antibody|GRO antibody|GRO protein, alpha antibody|GRO-alpha(1-73) antibody|GRO-alpha(6-73) antibody|Gro1 antibody|GRO1 oncogene(melanoma growth stimulating activity, alpha) antibody|GRO1 oncogene(melanoma growth-stimulating activity) antibody|Gro1 oncogene antibody|GroA antibody|GROA_HUMAN antibody|Growth regulated protein alpha antibody|Growth regulated protein GRO antibody|KC antibody|KC chemokine, mouse, homolog of antibody|Melanoma growth stimulatory activity alpha antibody|Melanoma growth stimulatory activity antibody|Melanoma growth stimulatory activity, alpha antibody|MGSA a antibody|MGSA alpha antibody|MGSA antibody|MGSA-a antibody|N51 antibody|NAP 3 antibody|NAP-3 antibody|NAP3 antibody|Neutrophil activating protein 3 antibody|Neutrophil-activating protein 3 antibody|Platelet-derived growth factor-inducible protein KC antibody|Scyb 1 antibody|SCYB1 antibody|Secretory protein N51 antibody|Small inducible cytokine subfamily B, member 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-GRO alpha antibody, ASA-B0821, Western blotting
All lanes: Anti GRO alpha (ASA-B0821) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 11KD
Observed bind size: 11KD
All lanes: Anti GRO alpha (ASA-B0821) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 11KD
Observed bind size: 11KD
