More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| glutamate receptor, ionotropic, kainate 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human GRIK1 recombinant protein (Position: R271-I450). Human GRIK1 shares 88% and 94% amino acid (aa) sequences identity with mouse and rat GRIK1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GRIK1 |
Protein | Glutamate receptor ionotropic, kainate 1 |
Uniprot ID | P39086 |
Function | Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. |
Tissue Specificity | Most abundant in the cerebellum and the suprachiasmatic nuclei (SCN) of the hypothalamus. |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. |
Sequence Similarities | |
Aliases | EAA3 antibody|EEA3 antibody|Excitatory amino acid receptor 3 antibody|GLR5 antibody|GluR-5 antibody|GluR5 antibody|GluR6 antibody|GluR7 antibody|Glutamate receptor 5 antibody|Glutamate receptor antibody|Glutamate receptor ionotropic kainate 1 antibody|GRIK1 antibody|GRIK1_HUMAN antibody|Human glutamate receptor GLUR5 antibody|ionotropic kainate 1 antibody|OTTHUMP00000096569 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- GRIK1 antibody, ASA-B0820, Western blotting
All lanes: Anti GRIK1 (ASA-B0820) at 0.5ug/ml
WB: Recombinant Human GRIK1 Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
All lanes: Anti GRIK1 (ASA-B0820) at 0.5ug/ml
WB: Recombinant Human GRIK1 Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
Anti- GRIK1 antibody, ASA-B0820, Western blotting
All lanes: Anti GRIK1 (ASA-B0820) at 0.5ug/ml
Lane 1: Mouse Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: SHG Whole Cell Lysate at 40ug
Predicted bind size: 104KD
Observed bind size: 104KD
All lanes: Anti GRIK1 (ASA-B0820) at 0.5ug/ml
Lane 1: Mouse Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: SHG Whole Cell Lysate at 40ug
Predicted bind size: 104KD
Observed bind size: 104KD
