More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| glutamate receptor, ionotropic, AMPA 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human GRIA4 recombinant protein (Position: Q176-K350). Human GRIA4 shares 99% amino acid (aa) sequence identity with both mouse and rat GRIA4. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GRIA4 |
Protein | Glutamate receptor 4 |
Uniprot ID | P48058 |
Function | Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell projection, dendrite. Note: Interaction with CNIH2, CNIH3 and PRKCG promotes cell surface expression. |
Sequence Similarities | |
Aliases | AMPA 4 antibody|AMPA selective glutamate receptor 4 antibody|AMPA-selective glutamate receptor 4 antibody|AMPA4 antibody|GluA 4 antibody|GluA4 antibody|GluR 4 antibody|GluR D antibody|GluR-4 antibody|GluR-D antibody|GluR4 antibody|GLUR4C antibody|GLURD antibody|Glutamate receptor 4 antibody|Glutamate receptor ionotrophic AMPA 4 antibody|Glutamate receptor ionotropic antibody|GRIA 4 antibody|Gria4 antibody|GRIA4_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- GRIA4 antibody, ASA-B0819, Western blotting
All lanes: Anti GRIA4 (ASA-B0819) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 101KD
Observed bind size: 101KD
All lanes: Anti GRIA4 (ASA-B0819) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 101KD
Observed bind size: 101KD
