More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| glutamate receptor, ionotropic, AMPA 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human GRIA3 recombinant protein (Position: G29-M360). Human GRIA3 shares 99% amino acid (aa) sequence identity with both mouse and rat GRIA3. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GRIA3 |
Protein | Glutamate receptor 3 |
Uniprot ID | P42263 |
Function | Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Note: Interaction with CNIH2 and CNIH3 promotes cell surface expression. |
Sequence Similarities | Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA3 subfamily. |
Aliases | AMPA 3 antibody|AMPA selective glutamate receptor 3 antibody|AMPA-selective glutamate receptor 3 antibody|dJ1171F9.1 antibody|GluA3 antibody|GLUK3 antibody|GLUR 3 antibody|GLUR C antibody|GLUR K3 antibody|GluR-3 antibody|GluR-C antibody|GluR-K3 antibody|GLUR3 antibody|GLURC antibody|Glutamate receptor 3 antibody|Glutamate receptor C antibody|Glutamate Receptor Ionotrophic AMPA 3 antibody|Glutamate receptor ionotropic antibody|Glutamate receptor subunit 3 antibody|GRIA 3 antibody|Gria3 antibody|GRIA3_HUMAN antibody|GRM 3 antibody|Ionotrophic Glutamate Receptor antibody|Metabotropic glutamate receptor 3 precursor antibody|mGlu 3 antibody|MGLUR 3 antibody|MRX94 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- GRIA3 antibody, ASA-B0818, Western blotting
All lanes: Anti GRIA3 (ASA-B0818) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 101KD
Observed bind size: 101KD
All lanes: Anti GRIA3 (ASA-B0818) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 101KD
Observed bind size: 101KD
