More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| chemokine(C-C motif) receptor 10 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human GPR2/CCR10(312-327aa FLGLRFRQDLRRLLRG), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CCR10 |
Protein | C-C chemokine receptor type 10(C-C CKR-10/CC-CKR-10/CCR-10) |
Uniprot ID | P46092 |
Function | Receptor for chemokines SCYA27 and SCYA28. Subsequently transduces a signal by increasing the intracellular calcium ions level and stimulates chemotaxis in a pre-B cell line. |
Tissue Specificity | Expressed at high levels in adult testis, small intestine, fetal lung, fetal kidney. Weaker expression was observed in many other adult tissues including spleen, thymus, lymph node, Peyer patches, colon, heart, ovary, peripheral blood lymphocytes, thyroid and spinal cord. Also expressed by melanocytes, dermal fibroblasts, dermal microvascular endothelial cells. Also detected in T-cells and in skin-derived Langerhans cells. |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. |
Sequence Similarities | Belongs to the G-protein coupled receptor 1 family. |
Aliases | C C chemokine receptor type 10 antibody|C C CKR 10 antibody|C-C chemokine receptor type 10 antibody|C-C CKR-10 antibody|CC chemokine receptor 10 antibody|CC CKR 10 antibody|CC-CKR-10 antibody|CCR 10 antibody|CCR-10 antibody|CCR10 antibody|CCR10_HUMAN antibody|Chemokine(C C motif) receptor 10 antibody|G protein coupled receptor 2 antibody|G protein-coupled receptor 2 antibody|G-protein coupled receptor 2 antibody|GPR 2 antibody|GPR2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-GPR2/CCR10 antibody, ASA-B0807, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SW620 Cell Lysate
Lane 3: A549 Cell Lysate
Lane 4: MM231 Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: SW620 Cell Lysate
Lane 3: A549 Cell Lysate
Lane 4: MM231 Cell Lysate
Lane 5: SMMC Cell Lysate
