More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| GLI family zinc finger 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Gli3(40-57aa SNEDESPGQTYHRERRNA), different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GLI3 |
Protein | Transcriptional activator GLI3 |
Uniprot ID | P10071 |
Function | Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'. |
Tissue Specificity | Is expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. |
Sub-cellular localization | Nucleus. Cytoplasm. Cell projection, cilium. Note: GLI3FL is localized predominantly in the cytoplasm while GLI3R resides mainly in the nucleus. Ciliary accumulation requires the presence of KIF7 and SMO. Translocation to the nucleus is promoted by interaction with ZIC1. |
Sequence Similarities | Belongs to the GLI C2H2-type zinc-finger protein family. |
Aliases | ACLS antibody|DNA binding protein antibody|GCPS antibody|Gli 3 antibody|GLI family zinc finger 3 antibody|GLI Kruppel family member GLI 3 antibody|GLI Kruppel family member GLI3(Greig cephalopolysyndactyly syndrome) antibody|GLI Kruppel family member GLI3 antibody|GLI3 antibody|GLI3 C-terminally truncated form antibody|GLI3 form of 190 kDa antibody|GLI3 form of 83 kDa antibody|GLI3 full length protein antibody|GLI3-190 antibody|GLI3-83 antibody|GLI3_HUMAN antibody|GLI3FL antibody|Glioma associated oncogene family zinc finger 3 antibody|Oncogene GLI3 antibody|PAP A antibody|PAPA 1 antibody|PAPA antibody|PAPA1 antibody|PAPB antibody|PHS antibody|PPD IV antibody|PPDIV antibody|Transcriptional activator GLI3 antibody|Transcriptional repressor GLI3R antibody|Zinc finger protein GLI 3 antibody|Zinc finger protein GLI3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Gli3 antibody, ASA-B0790, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: A549 Cell Lysate
Lane 3: SW620 Cell Lysate
Lane 1: Rat Testis Tissue Lysate
Lane 2: A549 Cell Lysate
Lane 3: SW620 Cell Lysate
