More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| GLI family zinc finger 2 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Gli2(1444-1463aa MLYYYGQIHMYEQDGGLENL). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GLI2 |
Protein | Zinc finger protein GLI2 |
Uniprot ID | P10070 |
Function | Has a dual function as a transcriptional activator and a repressor of the hedgehog (Hh) pathway. May play a role during embryogenesis. |
Tissue Specificity | Isoform 1 and isoform 4 are expressed in HTLV- 1-infected T-cell lines (at protein level). Isoform 1 and isoform 2 are strongly expressed in HTLV-1-infected T-cell lines. Isoform 3 and isoform 4 are weakly expressed in HTLV-1-infected T-cell lines. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the GLI C2H2-type zinc-finger protein family. |
Aliases | Gli 2 antibody|GLI family zinc finger 2 antibody|GLI kruppel family member 2 antibody|GLI Kruppel family member GLI2 antibody|GLI2 antibody|GLI2_HUMAN antibody|Oncogene GLI2 antibody|Tax helper protein antibody|Tax helper protein 2 antibody|Tax responsive element 2 holding protein antibody|Tax responsive element 25 bp sequence binding protein antibody|THP 2 antibody|THP antibody|THP2 antibody|Zinc finger protein GLI2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Gli2 antibody, ASA-B0789, Western blotting
WB: Human Placenta Tissue Lysate
WB: Human Placenta Tissue Lysate
Anti-Gli2 antibody, ASA-B0789, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SKOV Cell Lysate
Lane 4: HT1080 Cell Lysate
Lane 5: A549 Cell Lysate
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SKOV Cell Lysate
Lane 4: HT1080 Cell Lysate
Lane 5: A549 Cell Lysate
