More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| colony stimulating factor 3 (granulocyte) | Polyclonal | IgG | Rabbit | Human, Mouse | ELISA, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E. coli-derived mouse G-CSF recombinant protein (Position: R47-A208). Mouse G-CSF shares 77.2% amino acid (aa) sequence identity with human G-CSF. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CSF3 |
Protein | Granulocyte colony-stimulating factor |
Uniprot ID | P09920 |
Function | Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. |
Tissue Specificity | |
Sub-cellular localization | Secreted. |
Sequence Similarities | |
Aliases | C17orf33 antibody|Colony stimulating factor 3 (granulocyte) antibody|CSF 3 antibody|CSF beta antibody|CSF3 antibody|CSF3_HUMAN antibody| CSF3OS antibody|Csfg antibody|Filgrastim antibody|G-CSF antibody|GCSA antibody|GCSF antibody|Granulocyte colony stimulating factor antibody|Granulocyte colony-stimulating factor antibody|Lenograstim antibody|Macrophage granulocyte inducer 2 antibody|MGC45931 antibody| MGI 2 antibody|Pluripoietin antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse | AssaySolutio's ECL kit |
| ELISA | 0.1-0.5μg/ml | Mouse Mouse | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- G-CSF antibody, ASA-B0743, Western blotting
All lanes: Anti G-CSF (ASA-B0743) at 0.5ug/ml
WB: HELA Whole Cell Lysate at 40ug
Predicted bind size: 22KD
Observed bind size: 22KD
All lanes: Anti G-CSF (ASA-B0743) at 0.5ug/ml
WB: HELA Whole Cell Lysate at 40ug
Predicted bind size: 22KD
Observed bind size: 22KD
