More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| guanylate binding protein 1, interferon-inducible | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human GBP1(566-589aa QKESRIMKNEIQDLQTKMRRRKAC). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GBP1 |
Protein | Interferon-induced guanylate-binding protein 1 |
Uniprot ID | P32455 |
Function | Hydrolyzes GTP to GMP in two consecutive cleavage reactions. Exhibits antiviral activity against influenza virus. Promote oxidative killing and deliver antimicrobial peptides to autophagolysosomes, providing broad host protection against different pathogen classes. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm. Golgi apparatus membrane; Lipid- anchor; Cytoplasmic side. Secreted. Note: Secreted from endothelial cells in the cerebrospinal fluid, upon bacterial challenge and independently of interferon-gamma induction. Golgi membrane localization requires isoprenylation and the presence of another IFN-gamma-induced factor. |
Sequence Similarities | Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3-type GTPase family. GB1 subfamily. |
Aliases | GBP 1 antibody|GBP-1 antibody|GBP1 antibody|GBP1_HUMAN antibody|GTP binding protein 1 antibody|GTP-binding protein 1 antibody|Guanine nucleotide binding protein 1 antibody|Guanine nucleotide-binding protein 1 antibody|Guanylate binding protein 1 antibody|Guanylate binding protein 1 interferon inducible 67kDa antibody|Guanylate binding protein 1 interferon inducible antibody|HuGBP 1 antibody|HuGBP-1 antibody|HuGBP1 antibody|Interferon induced guanylate binding protein 1 antibody|Interferon-induced guanylate-binding protein 1 antibody|OTTHUMP00000012352 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-GBP1 antibody, ASA-B0770, Western blotting
All lanes: Anti GBP1 (ASA-B0770) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 68KD
Observed bind size: 68KD
All lanes: Anti GBP1 (ASA-B0770) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 68KD
Observed bind size: 68KD
