More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| GATA binding protein 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human GATA2(46-60aa DVFFNHLDSQGNPYY), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GATA2 |
Protein | Endothelial transcription factor GATA-2 |
Uniprot ID | P23769 |
Function | Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'. |
Tissue Specificity | Endothelial cells. |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Contains 2 GATA-type zinc fingers. |
Aliases | DCML antibody|Endothelial transcription factor GATA 2 antibody|Endothelial transcription factor GATA-2 antibody|Endothelial transcription factor GATA2 antibody|FLJ45948 antibody|GATA 2 antibody|GATA Binding Protein 2 antibody|GATA-binding protein 2 antibody|Gata2 antibody|GATA2_HUMAN antibody|MGC2306 antibody|MONOMAC antibody|NFE 1B antibody|NFE1B antibody|OTTHUMP00000216240 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-GATA2 antibody, ASA-B0767, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human GATA2, 38.3KD(162aa tag+ M1-G200)
Lane 1: Recombinant Human GATA2 Protein 10ng
Lane 2: Recombinant Human GATA2 Protein 5ng
Lane 3: Recombinant Human GATA2 Protein 2.5ng
Recombinant Protein Detection Source: E.coli derived -recombinant Human GATA2, 38.3KD(162aa tag+ M1-G200)
Lane 1: Recombinant Human GATA2 Protein 10ng
Lane 2: Recombinant Human GATA2 Protein 5ng
Lane 3: Recombinant Human GATA2 Protein 2.5ng
