More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| golgi-associated, gamma adaptin ear containing, ARF binding protein 1; golgi-associated, gamma adaptin ear containing, ARF binding protein 2; golgi-associated, gamma adaptin ear containing, ARF binding protein 3 | Monoclonal | IgG | Mouse | Human, Mouse, Rat | ICC, WB | Ascites |
Immunogen |
| Synthetic gamma-tubulin peptide(amino acids 38-53), conjugated to KLH. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GGA1; GGA2; GGA3 |
Protein | ADP-ribosylation factor-binding protein GGA1; ADP-ribosylation factor-binding protein GGA2; ADP-ribosylation factor-binding protein GGA3 |
Uniprot ID | Human(Q9UJY5), Mouse(Q8R0H9), Rat(Q5FVF3), Human(Q9UJY4), Mouse(Q6P5E6), Rat(Q4G038), Human(Q9NZ52), Mouse(Q8BMI3), Rat(D3ZHG8) |
Function | Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF- dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. |
Tissue Specificity | Ubiquitously expressed. |
Sub-cellular localization | Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. |
Sequence Similarities | Belongs to the GGA protein family. |
Aliases | VEAR|KIAA1080|KIAA0154|Gamma-adaptin-related protein 1|Gamma-adaptin-related protein 2|Golgi-localized, gamma ear-containing, ARF-binding protein 2|VHS domain and ear domain of gamma-adaptin |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.25-0.5μg/ml | Human, mouse, rat | AssaySolutio's ECL kit |
| Immunocytochemistry | Suitable | Human, mouse, rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for ICC. *Blocking peptide can be purchased at $65. Contact us for more information
