More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| gamma-aminobutyric acid (GABA) A receptor, beta 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human GABRB3 (344-375aa EKTAKAKNDRSKSESNRVDAHGNILLTSLEVH), different from the related mouse and rat sequences by five amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | GABRB3 |
Protein | Gamma-aminobutyric acid receptor subunit beta-3 |
Uniprot ID | P28472 |
Function | Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand- gated chloride channel. |
Tissue Specificity | |
Sub-cellular localization | Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. |
Sequence Similarities | Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB3 sub-subfamily. |
Aliases | ECA5 antibody|GABA A receptor beta 3 subunit antibody|GABA A receptor subunit beta 3 antibody|GABA alpha receptor beta 3 subunit antibody| GABA alpha receptor beta-2 subunit antibody|GABA(A) receptor beta 3 subunit antibody|GABA(A) receptor subunit beta 3 antibody|GABA(A) receptor subunit beta-3 antibody|GABAA receptor beta 3 subunit antibody|GABAA receptor subunit beta 3 antibody|GABR B3 antibody|GABRB 3 antibody|GABRB3 antibody|Gamma aminobutyric acid (GABA) A receptor beta 3 antibody|Gamma aminobutyric acid A receptor beta 3 antibody| Gamma aminobutyric acid receptor subunit beta 3 antibody|Gamma-aminobutyric acid receptor subunit beta-3 antibody|GBRB3_HUMAN antibody|MGC9051 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- GABRB3 antibody, ASA-B0748, Western blotting
All lanes: Anti GABRB3 (ASA-B0748) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 54KD
Observed bind size: 54KD
All lanes: Anti GABRB3 (ASA-B0748) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 54KD
Observed bind size: 54KD
