More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| furin (paired basic amino acid cleaving enzyme) | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human Furin recombinant protein (Position: T591-L794). Human Furin shares 88% amino acid (aa) sequence identity with both mouse and rat Furin. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FURIN |
Protein | Furin |
Uniprot ID | P09958 |
Function | Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. |
Tissue Specificity | Seems to be expressed ubiquitously. |
Sub-cellular localization | Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Note: Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin. |
Sequence Similarities | Belongs to the peptidase S8 family. Furin subfamily. |
Aliases | Dibasic processing enzyme antibody|Dibasic-processing enzyme antibody|FES upstream region antibody|FUR antibody|FURIN antibody|Furin membrane associated receptor protein antibody|FURIN_HUMAN antibody|PACE antibody|Paired basic amino acid residue cleaving enzyme antibody|Paired basic amino acid residue-cleaving enzyme antibody|PCSK3 antibody|Proprotein convertase subtilisin/kexin type 3 antibody|SPC1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Furin antibody, ASA-B0739, Western blotting
All lanes: Anti Furin (ASA-B0739) at 0.5ug/ml
WB: Recombinant Human Furin Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
All lanes: Anti Furin (ASA-B0739) at 0.5ug/ml
WB: Recombinant Human Furin Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
Anti- Furin antibody, ASA-B0739, Western blotting
All lanes: Anti Furin (ASA-B0739) at 0.5ug/ml
Lane 1: Hela Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: Colo320 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 87KD
Observed bind size: 87KD
All lanes: Anti Furin (ASA-B0739) at 0.5ug/ml
Lane 1: Hela Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: Colo320 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 87KD
Observed bind size: 87KD
