More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| frizzled-related protein | Polyclonal | IgG | Rabbit | Human | ELISA, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human FRZB recombinant protein (Position: E176-N325). Human FRZB shares 90% amino acid (aa) sequence identity with mouse FRZB. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FRZB |
Protein | Secreted frizzled-related protein 3 |
Uniprot ID | Q92765 |
Function | Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development. |
Tissue Specificity | Expressed primarily in the cartilaginous cores of the long bone during embryonic and fetal development and in the appendicular skeleton (6-13 weeks). At 13 weeks of gestation, transcripts were present in early chondroblasts of the tarsal bones of the foot, the carpal bones of the hands and the epiphysis of long bones. Highly expressed in placenta and heart, followed by brain, skeletal muscle, kidney and pancreas. Weakly expressed in lung and liver. |
Sub-cellular localization | Secreted . |
Sequence Similarities | Belongs to the secreted frizzled-related protein (sFRP) family. |
Aliases | FIZ antibody|FRE antibody|Frezzled antibody|Fritz antibody|Frizzled-related protein 1 antibody|FRP antibody|FrzB 1 antibody|FRZB antibody|FRZB1 antibody| OTTMUSP00000014350 antibody|RP23-33L19.1 antibody|Secreted frizzled related protein 3 [Precursor] antibody|Secreted frizzled related sequence protein 3 antibody|sFRP 3 antibody|SFRP antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- FRZB antibody, ASA-B0736, Western blotting
All lanes: Anti FRZB (ASA-B0736) at 0.5ug/ml
Lane 1: PANC Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
All lanes: Anti FRZB (ASA-B0736) at 0.5ug/ml
Lane 1: PANC Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
