More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| FOS-like antigen 2 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human FRA2 (96-127aa ALPRPGVIKTIGTTVGRRRRDEQLSPEEEEKR), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FOSL2 |
Protein | Fos-related antigen 2 |
Uniprot ID | P15408 |
Function | Controls osteoclast survival and size. As a dimer with JUN, activates LIF transcription. Activates CEBPB transcription in PGE2-activated osteoblasts. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. |
Sequence Similarities | Belongs to the bZIP family. Fos subfamily. |
Aliases | FLJ23306 antibody|Fos L2 antibody|FOS like antigen 2 antibody|Fos related antigen 2 antibody|Fos-related antigen 2 antibody|FosL 2 antibody| FosL2 antibody|FOSL2_HUMAN antibody|FRA 2 antibody|FRA-2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-FRA2 antibody, ASA-B0734, Western blotting
All lanes: Anti FRA2 (ASA-B0734) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 35KD
Observed bind size: 47KD
All lanes: Anti FRA2 (ASA-B0734) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 35KD
Observed bind size: 47KD
