More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| forkhead box O1 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human FOXO1A recombinant protein (Position: N456-G655). Human FOXO1A shares 93% amino acid (aa) sequence identity with both mouse and rat FOXO1A. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FOXO1 |
Protein | Forkhead box protein O1 |
Uniprot ID | Q12778 |
Function | Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'- TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC and PCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1. Promotes neural cell death. Mediates insulin action on adipose tissue. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide- damaged DNA in beta-cells. Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner. |
Tissue Specificity | Ubiquitous. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Contains 1 fork-head DNA-binding domain. |
Aliases | FKH 1 antibody|FKH1 antibody|FKHR antibody|Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody|Forkhead box O1 antibody|Forkhead box protein O1 antibody|Forkhead box protein O1A antibody|Forkhead in rhabdomyosarcoma antibody|Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody|FoxO transcription factor antibody|foxo1 antibody|FOXO1_HUMAN antibody|FOXO1A antibody|OTTHUMP00000018301 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- FOXO1A antibody, ASA-B0729, Western blotting
All lanes: Anti FOXO1A(ASA-B0729) at 0.5ug/ml
WB: Recombinant Human FOXO1A Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti FOXO1A(ASA-B0729) at 0.5ug/ml
WB: Recombinant Human FOXO1A Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti- FOXO1A antibody, ASA-B0729, Western blotting
All lanes: Anti FOXO1A (ASA-B0729) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 70KD
Observed bind size: 70KD
All lanes: Anti FOXO1A (ASA-B0729) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 70KD
Observed bind size: 70KD
