More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| flotillin 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Flotillin 2(117-131aa TLTVEQIYQDRDQFA), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FLOT2 |
Protein | Flotillin-2 |
Uniprot ID | Q14254 |
Function | May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function. |
Tissue Specificity | In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes. |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | Belongs to the band 7/mec-2 family. Flotillin subfamily. |
Aliases | ECS-1 antibody|ECS1 antibody|Epidermal surface antigen 1 antibody|Epidermal surface antigen antibody|ESA antibody|ESA1 antibody|Flot2 antibody|FLOT2_HUMAN antibody|Flotillin-2 antibody|M17S1 antibody|Membrane component chromosome 17 surface marker 1 antibody|Membrane component chromosome 17 surface marker 1 homolog antibody|Membrane component, chromosome 17, surface marker 1 antibody|REG-1 antibody|Reggie-1 antibody|Reggie-2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Flotillin 2 antibody, ASA-B0719, Western blotting
Lane 1: Rat Lung Tissue Lysate
Lane 2: 293T Cell Lysate
Lane 1: Rat Lung Tissue Lysate
Lane 2: 293T Cell Lysate
